Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function
Identifieur interne : 006A42 ( Main/Exploration ); précédent : 006A41; suivant : 006A43Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function
Auteurs : Jay W. Shin ; Giorgia Jurisic ; Michael DetmarSource :
- Experimental Cell Research [ 0014-4827 ] ; 2008.
Abstract
Lymphatic vessels play an important role in the maintenance of tissue fluid homeostasis and in the transport of immune cells to lymph nodes, but they also serve as the major conduit for cancer metastasis to regional lymph nodes. However, the molecular mechanisms regulating these functions are poorly understood. Based on transcriptional profiling studies of cultured human dermal lymphatic (LEC) versus blood vascular endothelial cells (BEC), we found that dipeptidyl peptidase IV (DPPIV) mRNA and protein are much more strongly expressed by cultured lymphatic endothelium than by blood vascular endothelium that only expressed low levels of DPPIV in culture. The enzymatic cleavage activity of DPPIV was significantly higher in cultured LEC than in BEC. Differential immunofluorescence analyses of human organ tissue microarrays for DPPIV and several vascular lineage-specific markers revealed that DPPIV is also specifically expressed
Url:
DOI: 10.1016/j.yexcr.2008.07.024
PubMed: 18708048
PubMed Central: 3398155
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 002770
- to stream Pmc, to step Curation: 002769
- to stream Pmc, to step Checkpoint: 003941
- to stream Ncbi, to step Merge: 002E79
- to stream Ncbi, to step Curation: 002E79
- to stream Ncbi, to step Checkpoint: 002E79
- to stream Main, to step Merge: 006B45
- to stream Main, to step Curation: 006A42
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function</title><author><name sortKey="Shin, Jay W" sort="Shin, Jay W" uniqKey="Shin J" first="Jay W." last="Shin">Jay W. Shin</name></author><author><name sortKey="Jurisic, Giorgia" sort="Jurisic, Giorgia" uniqKey="Jurisic G" first="Giorgia" last="Jurisic">Giorgia Jurisic</name></author><author><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18708048</idno><idno type="pmc">3398155</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398155</idno><idno type="RBID">PMC:3398155</idno><idno type="doi">10.1016/j.yexcr.2008.07.024</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">002770</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002770</idno><idno type="wicri:Area/Pmc/Curation">002769</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002769</idno><idno type="wicri:Area/Pmc/Checkpoint">003941</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003941</idno><idno type="wicri:Area/Ncbi/Merge">002E79</idno><idno type="wicri:Area/Ncbi/Curation">002E79</idno><idno type="wicri:Area/Ncbi/Checkpoint">002E79</idno><idno type="wicri:doubleKey">0014-4827:2008:Shin J:lymphatic:specific:expression</idno><idno type="wicri:Area/Main/Merge">006B45</idno><idno type="wicri:Area/Main/Curation">006A42</idno><idno type="wicri:Area/Main/Exploration">006A42</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function</title><author><name sortKey="Shin, Jay W" sort="Shin, Jay W" uniqKey="Shin J" first="Jay W." last="Shin">Jay W. Shin</name></author><author><name sortKey="Jurisic, Giorgia" sort="Jurisic, Giorgia" uniqKey="Jurisic G" first="Giorgia" last="Jurisic">Giorgia Jurisic</name></author><author><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name></author></analytic><series><title level="j">Experimental Cell Research</title><idno type="ISSN">0014-4827</idno><idno type="eISSN">1090-2422</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Lymphatic vessels play an important role in the maintenance of tissue fluid homeostasis and in the transport of immune cells to lymph nodes, but they also serve as the major conduit for cancer metastasis to regional lymph nodes. However, the molecular mechanisms regulating these functions are poorly understood. Based on transcriptional profiling studies of cultured human dermal lymphatic (LEC) versus blood vascular endothelial cells (BEC), we found that dipeptidyl peptidase IV (DPPIV) mRNA and protein are much more strongly expressed by cultured lymphatic endothelium than by blood vascular endothelium that only expressed low levels of DPPIV in culture. The enzymatic cleavage activity of DPPIV was significantly higher in cultured LEC than in BEC. Differential immunofluorescence analyses of human organ tissue microarrays for DPPIV and several vascular lineage-specific markers revealed that DPPIV is also specifically expressed <italic>in situ</italic> by lymphatic vessels of the skin, esophagus, small intestine, breast and ovary. Moreover, siRNA-mediated DPPIV knockdown inhibited LEC adhesion to collagen type I and to fibronectin, and also reduced cell migration and formation of tube-like structures. These results identify DPPIV as a novel lymphatic marker and mediator of lymphatic endothelial cell functions.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name><name sortKey="Jurisic, Giorgia" sort="Jurisic, Giorgia" uniqKey="Jurisic G" first="Giorgia" last="Jurisic">Giorgia Jurisic</name><name sortKey="Shin, Jay W" sort="Shin, Jay W" uniqKey="Shin J" first="Jay W." last="Shin">Jay W. Shin</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006A42 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 006A42 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3398155
|texte= Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18708048" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |